Composite

Part:BBa_K2461004:Design

Designed by: Nicholas J Kite   Group: iGEM17_UNebraska-Lincoln   (2017-10-17)


nrfHA Composite Part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 581
    Illegal BglII site found at 919
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 948
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silent mutations were made to avoid illegal restriction enzyme sites. 6xHis tags were added to the end of each coding sequence.


Source

The source of the coding sequences is Selenomonas ruminantium subsp. lactilytica TAM6421 registered on GenBank.

References